Skip to content
Get started

High-Throughput Mechanoporation Using Certus Flex Integration

image (9) 3

Watch the Replay: From Impermeable to Accessible

See how Portal's Galaxy mechanoporation technology integrates with the Certus Flex dispenser to enable high-throughput intracellular delivery—without sacrificing cell viability or workflow speed.

In this webinar, you'll learn:

  • How mechanoporation enables screening workflows with peptides and cell-impermeable small molecules
  • Real-time demonstration of the Galaxy + Certus Flex integrated workflow
  • Data showing 74% gene editing efficiency in unstimulated primary T cells with <5% well-to-well variation across 100+ dispenses
  • How parallel multi-cargo delivery (CRISPR + RNA in the same run) simplifies complex experiments

Why High-Throughput Mechanoporation?

Traditional delivery methods force a tradeoff: electroporation damages fragile primary cells, while lipofection simply doesn't work for many cargo types. Mechanoporation sidesteps these limitations by using gentle mechanical disruption to deliver cargo directly to the cytosol—preserving viability while opening access to previously impossible assays.

Integrate without disruption. Portal cartridges work in-line with the Certus Flex, requiring no changes to dispensing speed. Pre-load your cargo plates, dispense, and go. The entire 96-well plate processes in under a minute.

Consistent results at scale. Validated data across wells shows delivery efficiency remains stable plate-to-plate, with >80% delivery at 24 hours post-processing and <10% well-to-well CV. Your screening data stays clean.

Unlock new biology. Finally run functional assays with compounds that couldn't cross the membrane before—impermeable inhibitors, large proteins, even DNA-encoded libraries delivered directly into live primary cells.

Explore Applications